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A threonine to alanine exchange at position 40 of Tet repressor alters the recognition of the sixth base pair of tet operator from GC to AT.

The tet operators of two naturally evolved tetracycline resistance determinants differ by a G.C to A.T transition at the sixth base pair. This mutation prevents heterologous recognition of these tet operators by their respective two Tet repressor proteins. The amino acid side chains responsible for...

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Detalhes bibliográficos
Main Authors: Altschmied, L, Baumeister, R, Pfleiderer, K, Hillen, W
Formato: Artigo
Idioma:Inglês
Publicado em: 1988
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC455013/
https://ncbi.nlm.nih.gov/pubmed/3208760
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