Protein Conformational Changes Are Detected and Resolved Site Specifically by Second-Harmonic Generation

We present here a straightforward, broadly applicable technique for real-time detection and measurement of protein conformational changes in solution. This method is based on tethering proteins labeled with a second-harmonic generation (SHG) active dye to supported lipid bilayers. We demonstrate our...

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Bibliografski detalji
Izdano u:Biophys J
Glavni autori: Moree, Ben, Connell, Katelyn, Mortensen, Richard B., Liu, C. Tony, Benkovic, Stephen J., Salafsky, Joshua
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2015
Teme:
Proteins and Nucleic Acids
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4547196/
https://ncbi.nlm.nih.gov/pubmed/26287632
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2015.07.016
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