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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
High-density functional protein microarrays containing ~4,200 recombinant yeast proteins are examined for kinase protein-protein interactions using an affinity purified yeast kinase fusion protein containing a V5-epitope tag for read-out. Purified kinase is obtained through culture of a yeast strain...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4545172/ https://ncbi.nlm.nih.gov/pubmed/26274875 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51872 |
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