Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

High-density functional protein microarrays containing ~4,200 recombinant yeast proteins are examined for kinase protein-protein interactions using an affinity purified yeast kinase fusion protein containing a V5-epitope tag for read-out. Purified kinase is obtained through culture of a yeast strain...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Fasolo, Joseph, Im, Hogune, Snyder, Michael P.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2015
Subjects:
Cellular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4545172/
https://ncbi.nlm.nih.gov/pubmed/26274875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51872
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