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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
High-density functional protein microarrays containing ~4,200 recombinant yeast proteins are examined for kinase protein-protein interactions using an affinity purified yeast kinase fusion protein containing a V5-epitope tag for read-out. Purified kinase is obtained through culture of a yeast strain...
Αποθηκεύτηκε σε:
| Τόπος έκδοσης: | J Vis Exp |
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| Κύριοι συγγραφείς: | , , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
MyJove Corporation
2015
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4545172/ https://ncbi.nlm.nih.gov/pubmed/26274875 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51872 |
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