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Rate-determining Step of Flap Endonuclease 1 (FEN1) Reflects a Kinetic Bias against Long Flaps and Trinucleotide Repeat Sequences

Flap endonuclease 1 (FEN1) is a structure-specific nuclease responsible for removing 5′-flaps formed during Okazaki fragment maturation and long patch base excision repair. In this work, we use rapid quench flow techniques to examine the rates of 5′-flap removal on DNA substrates of varying length a...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Tarantino, Mary E., Bilotti, Katharina, Huang, Ji, Delaney, Sarah
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4543671/
https://ncbi.nlm.nih.gov/pubmed/26160176
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.666438
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