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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
Luminescent Identification of Functional Elements in 3’UTRs (3’LIFE) allows the rapid identification of targets of specific miRNAs within an array of hundreds of queried 3’UTRs. Target identification is based on the dual-luciferase assay, which detects binding at the mRNA level by measuring translat...
में बचाया:
| में प्रकाशित: | J Vis Exp |
|---|---|
| मुख्य लेखकों: | , , , |
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
MyJove Corporation
2015
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4542967/ https://ncbi.nlm.nih.gov/pubmed/26066857 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52647 |
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