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One Crystal, Two Temperatures: Cryocooling Penalties Alter Ligand Binding to Transient Protein Sites

Interrogating fragment libraries by X-ray crystallography is a powerful strategy for discovering allosteric ligands for protein targets. Cryocooling of crystals should theoretically increase the fraction of occupied binding sites and decrease radiation damage. However, it might also perturb protein...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Chembiochem
Egile Nagusiak: Fischer, Marcus, Shoichet, Brian K, Fraser, James S
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: WILEY-VCH Verlag 2015
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4539595/
https://ncbi.nlm.nih.gov/pubmed/26032594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201500196
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