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Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we ge...

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Bibliographic Details
Published in:Dev Cell
Main Authors: Lane, Andrew B., Strzelecka, Magdalena, Ettinger, Andreas, Grenfell, Andrew W., Wittmann, Torsten, Heald, Rebecca
Format: Artigo
Language:Inglês
Published: 2015
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4536113/
https://ncbi.nlm.nih.gov/pubmed/26212133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2015.06.003
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