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Enzymatically Generated CRISPR Libraries for Genome Labeling and Screening

CRISPR-based technologies have emerged as powerful tools to alter genomes and mark chromosomal loci, but an inexpensive method for generating large numbers of RNA guides for whole genome screening and labeling is lacking. Using a method that permits library construction from any source of DNA, we ge...

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Publicat a:Dev Cell
Autors principals: Lane, Andrew B., Strzelecka, Magdalena, Ettinger, Andreas, Grenfell, Andrew W., Wittmann, Torsten, Heald, Rebecca
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4536113/
https://ncbi.nlm.nih.gov/pubmed/26212133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.devcel.2015.06.003
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