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Top-down mass spectrometry of intact membrane protein complexes reveals oligomeric state and sequence information in a single experiment

Here we study the intact stoichiometry and top-down fragmentation behavior of three integral membrane proteins which were natively reconstituted into detergent micelles: the mechano-sensitive ion channel of large conductance (MscL), the Kirbac potassium channel and the p7 viroporin from the hepatiti...

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Detalhes bibliográficos
Publicado no:Protein Sci
Main Authors: Konijnenberg, Albert, Bannwarth, Ludovic, Yilmaz, Duygu, Koçer, Armağan, Venien-Bryan, Catherine, Sobott, Frank
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley & Sons, Ltd 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4534180/
https://ncbi.nlm.nih.gov/pubmed/25970171
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2703
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