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The impact of read length on quantification of differentially expressed genes and splice junction detection
BACKGROUND: The initial next-generation sequencing technologies produced reads of 25 or 36 bp, and only from a single-end of the library sequence. Currently, it is possible to reliably produce 300 bp paired-end sequences for RNA expression analysis. While read lengths have consistently increased, pe...
Enregistré dans:
| Publié dans: | Genome Biol |
|---|---|
| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
BioMed Central
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4531809/ https://ncbi.nlm.nih.gov/pubmed/26100517 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-015-0697-y |
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