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Poly Peak Parser: Method and software for identification of unknown indels using Sanger Sequencing of PCR products

BACKGROUND: Genome editing techniques, including ZFN, TALEN and CRISPR, have created a need to rapidly screen many F1 individuals to identify carriers of indels and determine the sequences of the mutations. Current techniques require multiple clones of the targeted region to be sequenced for each in...

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Detalhes bibliográficos
Publicado no:Dev Dyn
Main Authors: Hill, Jonathon T., Demarest, Bradley L., Bisgrove, Brent W., Su, Yi-chu, Smith, Megan, Yost, H. Joseph
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4525701/
https://ncbi.nlm.nih.gov/pubmed/25160973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/dvdy.24183
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