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ClickSeq: Fragmentation-free next-generation sequencing via click-ligation of adaptors to stochastically terminated 3’-azido cDNAs
We present a simple method called ‘ClickSeq’ for Next-Generation Sequencing (NGS) library synthesis that uses click-chemistry rather than enzymatic reactions for the ligation of Illumina sequencing adaptors. In ClickSeq, randomly-primed reverse transcription reactions are supplemented with azido-2’,...
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| Publié dans: | J Mol Biol |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2015
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4523409/ https://ncbi.nlm.nih.gov/pubmed/26116762 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.06.011 |
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