ClickSeq: Fragmentation-free next-generation sequencing via click-ligation of adaptors to stochastically terminated 3’-azido cDNAs

We present a simple method called ‘ClickSeq’ for Next-Generation Sequencing (NGS) library synthesis that uses click-chemistry rather than enzymatic reactions for the ligation of Illumina sequencing adaptors. In ClickSeq, randomly-primed reverse transcription reactions are supplemented with azido-2’,...

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Bibliografiske detaljer
Udgivet i:	J Mol Biol
Main Authors:	Routh, Andrew, Head, Steven R., Ordoukhanian, Phillip, Johnson, John E.
Format:	Artigo
Sprog:	Inglês
Udgivet:	2015
Fag:	Article
Online adgang:	https://ncbi.nlm.nih.gov/pmc/articles/PMC4523409/ https://ncbi.nlm.nih.gov/pubmed/26116762 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2015.06.011
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ClickSeq: Fragmentation-free next-generation sequencing via click-ligation of adaptors to stochastically terminated 3’-azido cDNAs

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