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Identification of contacts between topoisomerase I and its target DNA by site-specific photocrosslinking.

Vaccinia DNA topoisomerase, a eukaryotic type I enzyme, binds and cleaves duplex DNA at sites containing the sequence 5'-(T/C)CCTT. We report the identification of Tyr70 as the site of contact between the enzyme and the +4C base of its target site. This was accomplished by UV-crosslinking topoi...

Täydet tiedot

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Bibliografiset tiedot
Päätekijät: Sekiguchi, J, Shuman, S
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1996
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC451909/
https://ncbi.nlm.nih.gov/pubmed/8670847
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