Specific photocrosslinking of DNA-protein complexes: identification of contacts between integration host factor and its target DNA.
Azide moieties have been specifically placed in the backbone of DNA by chemical coupling between azidophenacyl bromide and uniquely positioned phosphorothioate residues. The derivatized DNA forms specific complexes with a DNA-binding protein and, following irradiation with 302-nm light, makes specif...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC45401/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7991603/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.91.25.12183 |
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