Identification of a contact between arginine-180 of the catabolite gene activator protein (CAP) and base pair 5 of the DNA site in the CAP-DNA complex.

We have used site-directed mutagenesis to replace amino acid 1 of the recognition alpha-helix of the catabolite gene activator protein (CAP), Arg-180, with glycine and with alanine. Substitution of Arg-180 of CAP eliminated specificity between G.C, A.T, C.G, and T.A at base pair 5 of the DNA half-si...

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Bibliographische Detailangaben
Hauptverfasser: Zhang, X P, Ebright, R H
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1990
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC54188/
https://ncbi.nlm.nih.gov/pubmed/2162054
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