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Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli

Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes remain uncertain. Antisense RNA (asRNA) silencing or in-frame gene disruptio...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Goh, Shan, Hohmeier, Angela, Stone, Timothy C., Offord, Victoria, Sarabia, Francisco, Garcia-Ruiz, Cristina, Good, Liam
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2015
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4510190/
https://ncbi.nlm.nih.gov/pubmed/26070674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01444-15
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