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Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli
Reliable methods for conditional gene silencing in bacteria have been elusive. To improve silencing by expressed antisense RNAs (asRNAs), we systematically altered several design parameters and targeted multiple reporter and essential genes in Escherichia coli. A paired termini (PT) design, where fl...
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| Autores principales: | , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1635301/ https://ncbi.nlm.nih.gov/pubmed/17062631 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl697 |
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