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Silencing of Essential Genes within a Highly Coordinated Operon in Escherichia coli

Essential bacterial genes located within operons are particularly challenging to study independently because of coordinated gene expression and the nonviability of knockout mutants. Essentiality scores for many operon genes remain uncertain. Antisense RNA (asRNA) silencing or in-frame gene disruptio...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Goh, Shan, Hohmeier, Angela, Stone, Timothy C., Offord, Victoria, Sarabia, Francisco, Garcia-Ruiz, Cristina, Good, Liam
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4510190/
https://ncbi.nlm.nih.gov/pubmed/26070674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01444-15
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