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Improved Gene Targeting through Cell Cycle Synchronization
Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell...
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| Publicado no: | PLoS One |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4507847/ https://ncbi.nlm.nih.gov/pubmed/26192309 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0133434 |
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