A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion

The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage...

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Bibliographic Details
Published in:J Virol
Main Authors: Li, Wentao, Wicht, Oliver, van Kuppeveld, Frank J. M., He, Qigai, Rottier, Peter J. M., Bosch, Berend-Jan
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2015
Subjects:
Virus-Cell Interactions
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4505673/
https://ncbi.nlm.nih.gov/pubmed/25972540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00356-15
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