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A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion

The emerging porcine epidemic diarrhea virus (PEDV) requires trypsin supplementation to activate its S protein for membrane fusion and virus propagation in cell culture. By substitution of a single amino acid in the S protein, we created a recombinant PEDV with an artificial furin protease cleavage...

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Библиографические подробности
Опубликовано в: :J Virol
Главные авторы: Li, Wentao, Wicht, Oliver, van Kuppeveld, Frank J. M., He, Qigai, Rottier, Peter J. M., Bosch, Berend-Jan
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4505673/
https://ncbi.nlm.nih.gov/pubmed/25972540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.00356-15
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