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Defining the enzyme binding domain of a ribonuclease III processing signal. Ethylation interference and hydroxyl radical footprinting using catalytically inactive RNase III mutants.

Ethylation interference and hydroxyl radical footprinting were used to identify substrate ribose-phosphate backbone sites that interact with the Escherichia coli RNA processing enzyme, ribonuclease III. Two RNase III mutants were employed, which bind substrate in vitro similarly as wild-type enzyme,...

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Bibliografski detalji
Izdano u:EMBO J
Glavni autori: Li, H, Nicholson, A W
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group 1996
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC450047/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8635475/
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