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Integration of Fourier Transform Infrared Spectroscopy, Fluorescence Spectroscopy, Steady-state Kinetics and Molecular Dynamics Simulations of Gα(i1) Distinguishes between the GTP Hydrolysis and GDP Release Mechanism

Gα subunits are central molecular switches in cells. They are activated by G protein-coupled receptors that exchange GDP for GTP, similar to small GTPase activation mechanisms. Gα subunits are turned off by GTP hydrolysis. For the first time we employed time-resolved FTIR difference spectroscopy to...

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Vydáno v:J Biol Chem
Hlavní autoři: Schröter, Grit, Mann, Daniel, Kötting, Carsten, Gerwert, Klaus
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Biochemistry and Molecular Biology 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4498047/
https://ncbi.nlm.nih.gov/pubmed/25979337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M115.651190
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