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Exploring Weak Ligand–Protein Interactions by Long-Lived NMR States: Improved Contrast in Fragment-Based Drug Screening**

Ligands that have an affinity for protein targets can be screened very effectively by exploiting favorable properties of long-lived states (LLS) in NMR spectroscopy. In this work, we describe the use of LLS for competitive binding experiments to measure accurate dissociation constants of fragments t...

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Detalhes bibliográficos
Publicado no:Angew Chem Int Ed Engl
Main Authors: Buratto, Roberto, Mammoli, Daniele, Chiarparin, Elisabetta, Williams, Glyn, Bodenhausen, Geoffrey
Formato: Artigo
Idioma:Inglês
Publicado em: WILEY-VCH Verlag 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4497601/
https://ncbi.nlm.nih.gov/pubmed/25196717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201404921
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