CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

BACKGROUND: One of the bottlenecks in production of biochemicals and pharmaceuticals in Saccharomyces cerevisiae is stable and homogeneous expression of pathway genes. Integration of genes into the genome of the production organism is often a preferred option when compared to expression from episoma...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Ronda, Carlotta, Maury, Jérôme, Jakočiu̅nas, Tadas, Baallal Jacobsen, Simo Abdessamad, Germann, Susanne Manuela, Harrison, Scott James, Borodina, Irina, Keasling, Jay D, Jensen, Michael Krogh, Nielsen, Alex Toftgaard
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4492099/
https://ncbi.nlm.nih.gov/pubmed/26148499
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0288-3
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