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Quantifying protein-mRNA interactions in single live cells

Specific binding proteins are crucial for the correct spatiotemporal expression of mRNA. To understand this process, a method is required to characterize RNA-protein interactions in single living cells with subcellular resolution. We combined endogenous single RNA and protein detection with two-phot...

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Publicat a:Cell
Autors principals: Wu, Bin, Buxbaum, Adina R., Katz, Zachary B., Yoon, Young J., Singer, Robert H.
Format: Artigo
Idioma:Inglês
Publicat: 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4491145/
https://ncbi.nlm.nih.gov/pubmed/26140598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cell.2015.05.054
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