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Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

An optimized method, based on the coupling of two commercial kits, is described for the extraction of soil nucleic acids, with simultaneous extraction and purification of DNA and RNA following a cascade scheme and avoiding the use of harmful solvents. The protocol canmonitor the variations in the re...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:MethodsX
Hauptverfasser: Tournier, E., Amenc, L., Pablo, A.L., Legname, E., Blanchart, E., Plassard, C., Robin, A., Bernard, L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2015
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4487712/
https://ncbi.nlm.nih.gov/pubmed/26150987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.03.007
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