Modification of a commercial DNA extraction kit for safe and rapid recovery of DNA and RNA simultaneously from soil, without the use of harmful solvents

An optimized method, based on the coupling of two commercial kits, is described for the extraction of soil nucleic acids, with simultaneous extraction and purification of DNA and RNA following a cascade scheme and avoiding the use of harmful solvents. The protocol canmonitor the variations in the re...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:MethodsX
Egile Nagusiak: Tournier, E., Amenc, L., Pablo, A.L., Legname, E., Blanchart, E., Plassard, C., Robin, A., Bernard, L.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Elsevier 2015
Gaiak:
Agricultural and Biological Science
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC4487712/
https://ncbi.nlm.nih.gov/pubmed/26150987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2015.03.007
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