MICROSPHERE-BASED FLOW CYTOMETRY PROTEASE ASSAYS FOR USE IN PROTEASE ACTIVITY DETECTION AND HIGH-THROUGHPUT SCREENING

This protocol describes microsphere-based protease assays for use in flow cytometry and high-throughput screening. This platform measures a loss of fluorescence from the surface of a microsphere due to the cleavage of an attached fluorescent protease substrate by a suitable protease enzyme. The assa...

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Vydáno v:Curr Protoc Cytom
Hlavní autoři: Saunders, Matthew J., Edwards, Bruce S., Zhu, Jingshu, Sklar, Larry A., Graves, Steven W.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2010
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4486899/
https://ncbi.nlm.nih.gov/pubmed/20938917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142956.cy1312s54
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