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SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
BACKGROUND: There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays t...
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| Udgivet i: | BMC Biotechnol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
BioMed Central
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4482279/ https://ncbi.nlm.nih.gov/pubmed/26112401 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0169-1 |
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