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SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR

BACKGROUND: There is a great interest in studying phosphotyrosine dependent protein-protein interactions in tyrosine kinase pathways that play a critical role in many aspects of cellular function. We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays t...

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Detalhes bibliográficos
Publicado no:BMC Biotechnol
Main Authors: Thompson, Christopher M., Bloom, Lee R., Ogiue-Ikeda, Mari, Machida, Kazuya
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4482279/
https://ncbi.nlm.nih.gov/pubmed/26112401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-015-0169-1
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