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EnD-Seq and AppEnD: sequencing 3′ ends to identify nontemplated tails and degradation intermediates

Existing methods for detecting RNA intermediates resulting from exonuclease degradation are low-throughput and laborious. In addition, mapping the 3′ ends of RNA molecules to the genome after high-throughput sequencing is challenging, particularly if the 3′ ends contain post-transcriptional modifica...

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Detalhes bibliográficos
Publicado no:RNA
Main Authors: Welch, Joshua D., Slevin, Michael K., Tatomer, Deirdre C., Duronio, Robert J., Prins, Jan F., Marzluff, William F.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2015
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4478355/
https://ncbi.nlm.nih.gov/pubmed/26015596
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.048785.114
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