Development of a nanobody-alkaline phosphatase fusion protein and its application in a highly sensitive direct competitive fluorescence enzyme immunoassay for detection of ochratoxin A in cereal

A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for ochratoxin A (OTA) based on a nanobody (Nb)-alkaline phosphatase (AP) fusion protein was developed. The VHH gene of Nb28 was subcloned into the expression vector pecan45 containing the AP double mutant gene. The N...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Anal Chem
Prif Awduron: Liu, Xing, Xu, Yang, Wan, De-bin, Xiong, Yong-hua, He, Zhen-yun, Wang, Xian-xian, Gee, Shirley J., Ryu, Dojin, Hammock, Bruce D.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2015
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4476795/
https://ncbi.nlm.nih.gov/pubmed/25531426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac504305z
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