Development of a nanobody-alkaline phosphatase fusion protein and its application in a highly sensitive direct competitive fluorescence enzyme immunoassay for detection of ochratoxin A in cereal

A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for ochratoxin A (OTA) based on a nanobody (Nb)-alkaline phosphatase (AP) fusion protein was developed. The VHH gene of Nb28 was subcloned into the expression vector pecan45 containing the AP double mutant gene. The N...

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Veröffentlicht in:Anal Chem
Hauptverfasser: Liu, Xing, Xu, Yang, Wan, De-bin, Xiong, Yong-hua, He, Zhen-yun, Wang, Xian-xian, Gee, Shirley J., Ryu, Dojin, Hammock, Bruce D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2015
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4476795/
https://ncbi.nlm.nih.gov/pubmed/25531426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac504305z
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