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Development of a Nanobody-Alkaline Phosphatase Fusion Protein and Its Application in a Highly Sensitive Direct Competitive Fluorescence Enzyme Immunoassay for Detection of 3-Phenoxybenzoic Acid in Urine

3-Phenoxybenzoic acid (3-PBA), 1(Figure 1) is a human urinary metabolite of many pyrethroid insecticides and can be used as a biomarker to monitor human exposure to these pesticides. A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for 3-PBA based on a nanobody (Nb)...

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Detalhes bibliográficos
Publicado no:J Agric Food Chem
Main Authors: Huo, Jingqian, Li, Zhenfeng, Wan, Debin, Li, Dongyang, Qi, Meng, Barnych, Bogdan, Vasylieva, Natalia, Zhang, Jinlin, Hammock, Bruce D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6442738/
https://ncbi.nlm.nih.gov/pubmed/30293433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.jafc.8b04521
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