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Subsecond Analyses of G Protein Couple Receptor Ternary Complex Dynamics by Rapid Mix Flow Cytometry
The binding of full and partial agonist ligands (L) to G protein coupled receptors (GPCRs) initiates the formation of ternary complexes with G proteins (LRG complexes). We describe the assembly of detergent solubilized LRG complexes on beads. Rapid mix flow cytometry is used to analyze the subsecond...
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| Izdano u: | Methods Enzymol |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2009
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4476792/ https://ncbi.nlm.nih.gov/pubmed/19480922 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/S0076-6879(09)05411-1 |
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