Llwytho...

Rapid parallel flow cytometry assays of active GTPases using effector beads

We describe a rapid assay for measuring the cellular activity of small GTPases in response to a specific stimulus. Effector functionalized beads are used to quantify in parallel multiple, GTP-bound GTPases in the same cell lysate by flow cytometry. In a biologically relevant example, five different...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Buranda, Tione, BasuRay, Soumik, Swanson, Scarlett, Agola, Jacob, Bondu, Virginie, Wandinger-Ness, Angela
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2013
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3848308/
https://ncbi.nlm.nih.gov/pubmed/23928044
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.07.039
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