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Rapid parallel flow cytometry assays of active GTPases using effector beads
We describe a rapid assay for measuring the cellular activity of small GTPases in response to a specific stimulus. Effector functionalized beads are used to quantify in parallel multiple, GTP-bound GTPases in the same cell lysate by flow cytometry. In a biologically relevant example, five different...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3848308/ https://ncbi.nlm.nih.gov/pubmed/23928044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.07.039 |
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