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Inducing RNAi in Drosophila Cells by Transfection with dsRNA

In Drosophila cells, RNA interference (RNAi) can be triggered by synthetic long double-stranded RNAs (dsRNAs). For many Drosophila cell lines and cell types, passive dsRNA uptake is inefficient. More complete silencing responses can often be obtained in Drosophila S2 cells using transfection, perhap...

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Bibliografiska uppgifter
I publikationen:Cold Spring Harb Protoc
Huvudupphovsmän: Zhou, Rui, Mohr, Stephanie, Hannon, Gregory J., Perrimon, Norbert
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2013
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4465107/
https://ncbi.nlm.nih.gov/pubmed/23637371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot074351
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