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Inducing RNAi in Drosophila Cells by Transfection with dsRNA

In Drosophila cells, RNA interference (RNAi) can be triggered by synthetic long double-stranded RNAs (dsRNAs). For many Drosophila cell lines and cell types, passive dsRNA uptake is inefficient. More complete silencing responses can often be obtained in Drosophila S2 cells using transfection, perhap...

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Detalhes bibliográficos
Publicado no:Cold Spring Harb Protoc
Main Authors: Zhou, Rui, Mohr, Stephanie, Hannon, Gregory J., Perrimon, Norbert
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4465107/
https://ncbi.nlm.nih.gov/pubmed/23637371
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot074351
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