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Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum

A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The cry...

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Detaylı Bibliyografya
Yayımlandı:Acta Crystallogr F Struct Biol Commun
Asıl Yazarlar: Papageorgiou, Anastassios C., Li, Duochuan
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: International Union of Crystallography 2015
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4461330/
https://ncbi.nlm.nih.gov/pubmed/26057795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15006366
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