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Expression, purification and crystallization of a family 55 β-1,3-glucanase from Chaetomium thermophilum
A β-1,3-glucanase from the thermophilic fungus Chaetomium thermophilum was overexpressed in Pichia pastoris, purified and crystallized in the presence of 1.8 M sodium/potassium phosphate pH 6.8 as a precipitant. Data to 2.0 Å resolution were collected in-house at 293 K from a single crystal. The cry...
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| Publicado no: | Acta Crystallogr F Struct Biol Commun |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4461330/ https://ncbi.nlm.nih.gov/pubmed/26057795 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X15006366 |
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