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Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase

Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studie...

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主要な著者: Wakadkar, Sachin, Zhang, Li-Qing, Li, Duo-Chuan, Haikarainen, Teemu, Dhavala, Prathusha, Papageorgiou, Anastassios C.
フォーマット: Artigo
言語:Inglês
出版事項: International Union of Crystallography 2010
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935235/
https://ncbi.nlm.nih.gov/pubmed/20823534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110030393
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