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Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase
Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studie...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
International Union of Crystallography
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2935235/ https://ncbi.nlm.nih.gov/pubmed/20823534 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110030393 |
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