Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase

Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studie...

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Detaylı Bibliyografya
Asıl Yazarlar: Wakadkar, Sachin, Zhang, Li-Qing, Li, Duo-Chuan, Haikarainen, Teemu, Dhavala, Prathusha, Papageorgiou, Anastassios C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: International Union of Crystallography 2010
Konular:
Crystallization Communications
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935235/
https://ncbi.nlm.nih.gov/pubmed/20823534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110030393
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