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Expression, purification and crystallization of Chaetomium thermophilum Cu,Zn superoxide dismutase

Cu,Zn superoxide dismutase (Cu,ZnSOD) from the thermophilic fungus Chaetomium thermophilum was expressed in Pichia pastoris and purified. Crystals were grown in over 120 conditions but only those produced with 1.4 M sodium potassium phosphate pH 8.2 as precipitant were suitable for structural studie...

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Bibliografiska uppgifter
Huvudupphovsmän: Wakadkar, Sachin, Zhang, Li-Qing, Li, Duo-Chuan, Haikarainen, Teemu, Dhavala, Prathusha, Papageorgiou, Anastassios C.
Materialtyp: Artigo
Språk:Inglês
Publicerad: International Union of Crystallography 2010
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935235/
https://ncbi.nlm.nih.gov/pubmed/20823534
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110030393
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