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Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9

Genome editing, which introduces mutations in genes of interest using artificial DNA nucleases such as the ZFN, TALEN, and CRISPR/Cas9 systems in living cells, is a useful tool for generating mutant animals. Although CRISPR/Cas9 provides advantages over the two other systems, such as an easier vecto...

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Foilsithe in:Sci Rep
Main Authors: Hara, Satoshi, Tamano, Moe, Yamashita, Satoshi, Kato, Tomoko, Saito, Takeshi, Sakuma, Tetsushi, Yamamoto, Takashi, Inui, Masafumi, Takada, Shuji
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group 2015
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4460908/
https://ncbi.nlm.nih.gov/pubmed/26057433
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11221
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