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Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

Introducing a point mutation is a fundamental method used to demonstrate the roles of particular nucleotides or amino acids in the genetic elements or proteins, and is widely used in in vitro experiments based on cultured cells and exogenously provided DNA. However, the in vivo application of this a...

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Detalhes bibliográficos
Main Authors: Inui, Masafumi, Miyado, Mami, Igarashi, Maki, Tamano, Moe, Kubo, Atsushi, Yamashita, Satoshi, Asahara, Hiroshi, Fukami, Maki, Takada, Shuji
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066261/
https://ncbi.nlm.nih.gov/pubmed/24953798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05396
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