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Rapid generation of mouse models with defined point mutations by the CRISPR/Cas9 system

Introducing a point mutation is a fundamental method used to demonstrate the roles of particular nucleotides or amino acids in the genetic elements or proteins, and is widely used in in vitro experiments based on cultured cells and exogenously provided DNA. However, the in vivo application of this a...

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Détails bibliographiques
Auteurs principaux: Inui, Masafumi, Miyado, Mami, Igarashi, Maki, Tamano, Moe, Kubo, Atsushi, Yamashita, Satoshi, Asahara, Hiroshi, Fukami, Maki, Takada, Shuji
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4066261/
https://ncbi.nlm.nih.gov/pubmed/24953798
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep05396
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