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Online Peptide Fractionation Using a Multiphasic Microfluidic Liquid Chromatography Chip Improves Reproducibility and Detection Limits for Quantitation in Discovery and Targeted Proteomics

Comprehensive proteomic profiling of biological specimens usually requires multidimensional chromatographic peptide fractionation prior to mass spectrometry. However, this approach can suffer from poor reproducibility because of the lack of standardization and automation of the entire workflow, thus...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cell Proteomics
Hauptverfasser: Krisp, Christoph, Yang, Hao, van Soest, Remco, Molloy, Mark P
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2015
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4458731/
https://ncbi.nlm.nih.gov/pubmed/25850434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M114.046425
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