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Online Peptide Fractionation Using a Multiphasic Microfluidic Liquid Chromatography Chip Improves Reproducibility and Detection Limits for Quantitation in Discovery and Targeted Proteomics

Comprehensive proteomic profiling of biological specimens usually requires multidimensional chromatographic peptide fractionation prior to mass spectrometry. However, this approach can suffer from poor reproducibility because of the lack of standardization and automation of the entire workflow, thus...

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Библиографические подробности
Опубликовано в: :Mol Cell Proteomics
Главные авторы: Krisp, Christoph, Yang, Hao, van Soest, Remco, Molloy, Mark P
Формат: Artigo
Язык:Inglês
Опубликовано: The American Society for Biochemistry and Molecular Biology 2015
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC4458731/
https://ncbi.nlm.nih.gov/pubmed/25850434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M114.046425
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