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Online Peptide Fractionation Using a Multiphasic Microfluidic Liquid Chromatography Chip Improves Reproducibility and Detection Limits for Quantitation in Discovery and Targeted Proteomics

Comprehensive proteomic profiling of biological specimens usually requires multidimensional chromatographic peptide fractionation prior to mass spectrometry. However, this approach can suffer from poor reproducibility because of the lack of standardization and automation of the entire workflow, thus...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Main Authors: Krisp, Christoph, Yang, Hao, van Soest, Remco, Molloy, Mark P
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4458731/
https://ncbi.nlm.nih.gov/pubmed/25850434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M114.046425
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