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Method for trapping affinity chromatography of transcription factors using aldehyde-hydrazide coupling to agarose
The uses of a method of coupling DNA is investigated for trapping and purifying transcription factors. Using the GFP-C/EBP fusion protein as a model, trapping gives higher purity and comparable yield to conventional affinity chromatography. The chemistry utilized is mild and was shown to have no det...
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| Udgivet i: | Anal Biochem |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2015
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4458446/ https://ncbi.nlm.nih.gov/pubmed/25935261 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2015.04.025 |
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