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Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly Using Xenopus In Vitro Assays

The large and complex eukaryotic nucleus is the arbiter of DNA replication, RNA transcription, splicing, and ribosome assembly. With the advent of in vitro nuclear reconstitution extracts derived from Xenopus eggs in the 1980s, it became possible to assemble multiple nuclei in vitro around added DNA...

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Dades bibliogràfiques
Publicat a:Methods Cell Biol
Autors principals: Bernis, Cyril, Forbes, Douglass J.
Format: Artigo
Idioma:Inglês
Publicat: 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454467/
https://ncbi.nlm.nih.gov/pubmed/24857730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-417160-2.00008-4
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