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Analysis of Nuclear Reconstitution, Nuclear Envelope Assembly, and Nuclear Pore Assembly Using Xenopus In Vitro Assays

The large and complex eukaryotic nucleus is the arbiter of DNA replication, RNA transcription, splicing, and ribosome assembly. With the advent of in vitro nuclear reconstitution extracts derived from Xenopus eggs in the 1980s, it became possible to assemble multiple nuclei in vitro around added DNA...

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Détails bibliographiques
Publié dans:Methods Cell Biol
Auteurs principaux: Bernis, Cyril, Forbes, Douglass J.
Format: Artigo
Langue:Inglês
Publié: 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4454467/
https://ncbi.nlm.nih.gov/pubmed/24857730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/B978-0-12-417160-2.00008-4
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